High Throughput Determination of Stern Volmer Quenching Constants for Common Photocatalysts and Quenchers

03 March 2023, Version 1
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

Mechanistic information on reactions proceeding via photoredox catalysis has enabled rational optimizations of existing reactions and revealed new synthetic pathways. One essential step in any photoredox reaction is catalyst quenching via photoinduced electron transfer or energy transfer with either a substrate, additive, or co-catalyst. Identification of the correct quencher using Stern-Volmer studies is a necessary step mechanistic understanding; however, such studies are often cumbersome, low throughput and require specialized luminescence instruments. This report describes a high throughput method to rapidly acquire series of Stern-Volmer constants, employing readily available fluorescence plate readers and 96 well-plates. By leveraging multi-channel pipettors or liquid dispensing robots in combination with fast plate readers, the sampling frequency for quenching studies can be improved by several orders of magnitude. This new high-throughput method enabled the rapid collection of 220 quenching constants for a library of 20 common photocatalysts with 11 common quenchers. The extensive Stern-Volmer constants table generated greatly facilitates the systematic comparison between quenchers and can provide guidance to the synthetic community interested in designing and understanding catalytic photoredox reactions

Keywords

Photoredox catalysis
Stern-Volmer quenching
high throughput experimentation

Supplementary materials

Title
Description
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Title
Supporting information
Description
Plate design, data analysis and raw emission data
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Tecan code
Description
Robot handler procedure for solution dispensing
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