Quantitative Analysis of Diubiquitin Isomers Using Ion Mobility-Mass Spectrometry

13 January 2023, Version 1
This content is a preprint and has not undergone peer review at the time of posting.


The diversity of ubiquitin modifications calls for methods to better characterize ubiquitin chain linkage, length, and morphology. Here, we use multiple linear regression analysis coupled with ion mobility mass spectrometry (IM-MS) to quantify the relative abundance of different ubiquitin dimer isomers. We demonstrate the utility and robustness of this approach by quantifying the relative abundance of different ubiquitin dimers in complex mixtures and comparing the results to the standard, bottom-up ubiquitin AQUA method. Our results provide a foundation for using multiple linear regression analysis and IM-MS to characterize more complex ubiquitin chain architectures.


ion mobility
ubiquitin isoforms
quantitative analysis

Supplementary materials

Supporting Information
Supporting information contains Figures S1-S11, Tables S1-S2


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