The Decarboxylation and Protonation Enigma in the H85Q Mutant of Cytochrome P450OleT

12 January 2023, Version 1
This content is a preprint and has not undergone peer review at the time of posting.


Cytochrome P450OleT (CYP450OleT), a member of CYP450 Peroxygenases, catalyzes unusual decarboxylation activity. Unlike other members of the peroxygenases family, CYP450OleT possesses a Histidine at the 85th position, which was supposed to be the root cause of the decarboxylation activity in CYP450OleT. This work addresses the His85Gln mutant paradox where mutation of HisGln still shows efficient decarboxylation activity in CYP450OleT. The MD simulation of the H85Q mutant of CYP450OleT shows that in the absence of the histidine at 85th position, an Asp239 plays a similar role via a well-organized water channel. Our simulation shows that such a water channel is vital for the optimal substrate positioning needed for the decarboxylation activity and is gated by the Q85-R242 residue pair. Interestingly, the MD simulation of the WT CYP450BSβ shows a closed channel that blocks the access to the Glu236 (analogous residue to Asp239 in CYP450OleT) and, therefore CYP450BSβ shows low decarboxylation activity.


MD Simulations
QM/MM Calculations
Reaction Mechanism

Supplementary materials

Supporting Material
The supporting information contains the QM geometries, reaction profiles and RMSDs during the simulations


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