Folding double stranded DNA into designed shapes with triplex forming oligonucleotides

29 December 2022, Version 1
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

The folding of double-stranded DNA around histones is a central mechanism in eukaryotic cells for compacting the genetic information into chromosomes. Very few artificial methods are available for controlling the shape of dsDNA at any level, whereas several artificial methods have been developed to efficiently organize single-stranded DNA and RNA into a variety of well-defined nanostructures by programmed self-assembly , . Here, we show how long double-stranded DNA sequences can be spatially organized by triplex forming oligonucleotides (TFOs), which bridge two or more embedded polypurine domains. The linearized or plasmid dsDNA is compacted into antiparallel folds, which enables the formation of raster-filled 2D shapes and 3D structures with either square or hexagonal organizations. Contrary to ssDNA, dsDNA has inherent rigidity which alleviates the requirement to saturate a structure with TFO strands, yet the TFOs are still able to bend the dsDNA controllably and steeply up to 180° over 6 bp. The majority of structures investigated here are formed by Hoogsteen interactions which require pH = 5-6, however, the methodology is also applied with reverse Hoogsteen interactions at physiological pH. In both cases, the DNA triplexes render pure polypurine scaffolded structures resistant to DNase I.

Keywords

DNA Nanotechnology
Triplex
dsDNA
Origami
Hoogsteen

Supplementary materials

Title
Description
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Title
Supplementary Material I
Description
Methods, Supplementary Figures and Tables, and DNA sequences.
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Supplementary Material II
Description
Design Schemes
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