Wood from Angiosperms and Gymnosperms Shows Distinctive Lignin Peaks in Direct Analysis in Real Time (DART) Mass Spectra

02 December 2022, Version 2
This content is a preprint and has not undergone peer review at the time of posting.


A data set was constructed consisting of 3021 mass spectra randomly selected from the ForeST© (Forensic Spectra of Trees) database of mass spectra for wood analyzed by Direct Analysis in Real Time ionization coupled with time-of-flight mass spectrometry (DART-TOFMS). Clear and reproducible differences were observed between the lignin peaks for angiosperms and gymnosperms, with DART-TOFMS spectra of angiosperms showing significantly higher abundances for peaks associated with syringyl subunits. These differences can be used to provide support for enforcing trade laws by accurately identifying the source of finished wood products when anatomical differences are not present.


mass spectrometry
ambient ionization

Supplementary materials

Supporting Data for “Wood from Angiosperms and Gymnosperms shows Distinctive Lignin Peaks in Direct Analysis in Real Time (DART) Mass Spectra”.
Relative abundance of lignin peaks in angiosperms and gymnosperms; Principal Component Analysis using the lignin peaks to distinguish between angiosperms and gymnosperms in all 3021 mass spectra randomly selected from the ForeST© database; biplot showing how the compounds associated with the lignin peaks contribute to the PCA classification; confusion matrix showing the correct and incorrect assignments for the 803 DART mass spectra selected for method validation.


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