Comprehensive lipidome of human plasma using minimal sample manipulation by liquid chromatography coupled with mass spectrometry

07 November 2022, Version 3
This content is a preprint and has not undergone peer review at the time of posting.


Comprehensive analysis of the lipidome is addressed here by analysing lipid subclasses not easily detected by current high-throughput methods. Abundant lipid subclasses in human plasma are chromatographically separated from low abundance lipids prior to detection, avoiding the need for derivatisation. Lipid subclasses from the de novo lipogenesis and sphingolipids pathways are presented in this work. Three chromatographic methods here were implemented using a tertiary pumping system to allow for the inclusion of a gradient for analyte separation using A and B pumps, while an isocratic wash elutes interfering compounds. The isocratic wash enabled elution of lipid subclasses not targeted within the method that would otherwise cause background signal in the subsequent sample injection and reduction in column lifetime. Four chromatographic methods coupled with mass spectrometry using targeted and untargeted approaches to separate high and low abundance lipid subclasses are described here. An optimised method for the extraction of lysolipids is also used in addition to Folch extraction in human plasma.


lysophosphatidic acid
lipid analysis
lipid profile
human plasma
mass spectrometry
phosphatidic acid
lysolipids extraction method

Supplementary materials

Comprehensive lipidome of human plasma using 1 minimal sample manipulation by liquid chromatography coupled with mass spectrometry
Description of detailed protocols


Comments are not moderated before they are posted, but they can be removed by the site moderators if they are found to be in contravention of our Commenting Policy [opens in a new tab] - please read this policy before you post. Comments should be used for scholarly discussion of the content in question. You can find more information about how to use the commenting feature here [opens in a new tab] .
This site is protected by reCAPTCHA and the Google Privacy Policy [opens in a new tab] and Terms of Service [opens in a new tab] apply.