Orientation-dependent real-time single-molecule photobleaching inside uniform electrodynamic interfaces of nanofluidic confinement

25 October 2022, Version 1
This content is a preprint and has not undergone peer review at the time of posting.


The functioning of single molecules in nanofluidic confinement is a typical process in cell biology. The orientation of molecules is a critical parameter for this. We discern the orientation of a single molecule in a nanofluidic environment while it's functioning in an engineered solid-state device. Molecular properties depend on the electrodynamic interface. Our manufacturing ability of uniform electrodynamic interfaces at nanometric lengthscale opens the avenue of imitating biological abilities to handle single molecules with single-charge precision. We present a step-wise single-molecule fluorescence photobleaching study in a nanoconfined space of 25 nm to 45 nm. The uniform electrodynamics interfaces of silica-silica let us study the artefact-free dependence of molecular interface and its effect on step-wise photobleaching with a controlled environment of oxygen at room temperature.


single-molecule fluorescence
molecular orientation
defocused imaging


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