Abstract
3-Ketosteroid Δ1-dehydrogenases (KstD) are important microbial flavin enzymes that initiate the metabolism of steroid ring A and are useful find application in the synthesis of steroid drugs. We present a structure of the KstD from Sterolibacterium denitrificans (AcmB), which contains a previously uncharacterized membrane-associated domain and extended proton-relay system. The experimental and theoretical studies show that the steroid 1,2-dehydrogenation proceeds according to the
Ping-Pong bi-bi kinetics and a two-step base-assisted elimination (E2cB) mechanism. The mechanism is validated by evaluating the experimental and theoretical kinetic isotope effect for deuterium substituted substrates. The role of the active site residues is quantitatively assessed by point mutations, experimental activity assays, and QM/MM MD modeling of the reductive half-reaction (RHR). The pre-steady-state kinetics also reveals that the low pH (6.5) optimum of AcmB is dictated by the oxidative half-reaction (OHR), while the RHR exhibits a slight optimum at the pH usual for the KstD family of 8.5. Finally, the modeling confirms the origin of the enantioselectivity of C2-H activation and substrate specificity for Δ1-3-
ketosteroids.
Supplementary materials
Title
Supplementary Information
Description
Extended experimental procedure (Site-directed mutagenesis, Kinetic isotope effect– competition method, QMMM model setup), phylogenetic tree for ‘loop’ sequences of KstDs and six-tyrosine motif analysis, Diffraction data collection and refinement statistics, Size-exclusion chromatograms of AcmB, Figures and mobility analyses of Membrane-associated domain, figures of AcmB structure, binding sites, details on MMPBSA DGbinding and interaction energies for AcmB and KstD1, details on MD trajectories, figures of stationary states of all mechanisms and figures depicting PES profiles, detailed data on activities of mutants, experimental and theoretical kinetic isotope effects,
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Title
SI - population analysis
Description
Population analysis
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