Materials Science

Electrospun Polyacrylonitrile Silver Nanoparticle (PAN-AgNP) Nanocomposites as Alternative Antimicrobial Materials

Authors

  • William Wang Department of Scientific Research, Taipei American School ,
  • Chieh-Yu Pan Department and Graduate Institute of Aquaculture, National Kaohsiung University of Science and Technology ,
  • Eng-Yen Huang Department of Radiation Oncology, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine & School of Traditional Chinese Medicine, Chang Gung University ,
  • Bai-Jing Peng School of Pharmacy, College of Pharmacy, Kaohsiung Medical University ,
  • Jonathan Hsu Department of Scientific Research, Taipei American School ,
  • Jude Clapper Department of Scientific Research, Taipei American School

Abstract

Infectious microbial diseases can easily be transferred from person to person in the air or via high contact surfaces. As a result, researchers must aspire to create materials that can be implemented in surface contact applications to disrupt pathogen growth and transmission. Silver (Ag) is known to possess antimicrobial activity and has been used in the past in various pharmaceutical applications. Herein, we examine the antimicrobial properties of polyacrylonitrile (PAN) nanofibers coated with different concentrations of silver nanoparticles (AgNPs). Polyacrylonitrile (PAN) was homogenized with varied weight concentrations of silver nitrate (AgNO3) in N,N-Dimethylformamide (DMF) solution, a common organic solvent that serves as both an electrospinning solvent and as a reducing agent that forms AgNPs. The subsequent colloids were electrospun into nanofibers, which were then characterized via various analysis techniques, including Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Energy Dispersive X-Ray (EDX) Analysis, Dynamic Light Scattering (DLS), and X-Ray Photoelectron Spectroscopy (XPS). 10 microbes, including 7 strains of Gram-positive bacteria, 2 strains of Gram-negative bacteria, and Candida albicans were incubated with cutouts of various PAN-AgNP nanocomposites using disk diffusion methods to test for the nanocomposites’ antimicrobial efficiency. We report that PAN-AgNP nanocomposites retain a certain degree of antimicrobial longevity; samples stored for approximately 90 days demonstrate similar antimicrobial activity against Escherichia coli (E. coli) and Lactobacillus crispatus (L. crispatus) when compared to their newly electrospun counterparts. Moreover, our results indicate that PAN-AgNP nanocomposites successfully display antimicrobial activity against various bacteria and fungi strains regardless of their resistance to conventional antibiotics. Our study demonstrates that PAN-AgNP nanocomposites can potentially be applied to surfaces at risk of contracting microbial infections.

Version notes

Removed minor typos and finalized author contributions.

Content

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Supplementary material

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Supplementary Materials for Electrospun Polyacrylonitrile Silver Nanoparticle (PAN-AgNP) Nanocomposites as Alternative Antimicrobial Materials
Supplementary Materials for Electrospun Polyacrylonitrile Silver Nanoparticle (PAN-AgNP) Nanocomposites as Alternative Antimicrobial Materials including SEM EDX Spectrum, TEM EDX Spectrum, XPS Atomic Weight Quantifications, and Preserved versus New Nanofibers