Dissecting transmembrane bicarbonate transport by 1,8-di(thio)amidocarbazoles

09 September 2022, Version 2
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

Synthetic ionophores able to transport bicarbonate and chloride anions across lipid bilayers are appealing for their wide range of potential biological applications. We have studied the bicarbonate and chloride transport by carbazoles with two amido/thioamido groups using a bicarbonate-sensitive europium(III) probe in liposomes and found a highly remarkable concentration dependence. This can be explained by a combination of two distinct transport mechanisms: HCO3−/Cl− exchange and a combination of unassisted CO2 diffusion and HCl transport, of which the respective contributions were quantified. The compounds studied were found to be highly potent HCl transporters. Based on the mechanistic insights on anion transport, we have tested the antimicrobial activity of these compounds and found good correlation with their ion transport properties and a high activity against Gram-positive bacteria.

Keywords

anion transport
transport mechanisms
bicarbonate transport
chloride transport
synthetic anionophores
antibacterial properties

Supplementary materials

Title
Description
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Title
Dissecting transmembrane bicarbonate transport by 1,8-di(thio)amidocarbazoles
Description
A file containing detailed protocols and results of anion transport studies and biological studies.
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