Water-soluble Ionic Metal-Organic Polyhedra as a Versatile Platform for Enzyme Bio-immobilization

08 September 2022, Version 1
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

Metal-organic polyhedra (MOPs) can act as elementary structural units for the design of modular porous materials; however, their association with biological systems remains greatly restricted by their typically low stabilities and solubilities in water. Hereby, we describe the preparation of novel MOPs bearing either anionic or cationic groups and exhibiting a high affinity for proteins. Simple mixing of protein (BSA) and ionic MOPs aqueous solutions resulted in the spontaneous formation of MOP-protein assemblies, in a colloidal state or as solid precipitates depending on the initial mixing ratio. The versatility of the method was further illustrated using two enzymes, catalase and cytochrome c, with different sizes and isoelectric points (pI) below and above 7. This mode of assembly led to the high retention of catalytic activity and enabled recyclability. Furthermore, the co-immobilization of cytochrome c with highly charged MOPs resulted in a substantial 44-fold increase of its catalytic activity.

Keywords

cage
enzyme
microporous material
self-assembly

Supplementary materials

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Description
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Supplementary materials
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Additional methods and characterizations
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Video of the O2 evolution by a flake of RhPos-Cat
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Flake was immersed in 0.5 wt% H2O2. The bubbles are due to the catalytic decompositon of H2O2 in O2.
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