BODIPY-labelled estrogens for fluorescence analysis of environmental microbial degradation

28 July 2022, Version 2
This content is a preprint and has not undergone peer review at the time of posting.


Biodegradation of estrogen hormone micropollutants is a pervasive approach towards their remediation. Fluorescently labelled substrates are used extensively for rapid, near real-time analysis of biological processes and are a potential tool for studying biodegradation processes faster and more efficiently than conventional approaches. However, it is important to understand how the fluorescently tagged surrogates compare with the natural substrate in terms of chemical analysis and the intended application. We derivatized three natural estrogens with BODIPY fluorophores by azide-alkyne cycloaddition click reaction and developed an analytical workflow based on simple liquid-liquid extraction and HPLC-PDA analysis. The developed methods allow for concurrent analysis of both fluorescent and natural estrogens with comparable recovery, accuracy, and precision. We then evaluated the suitability of BODIPY-labelled estrogens as surrogate substrates for studying biodegradation using a model bacterium for estrogen metabolism. In service of this objective, the developed analytical methods were successfully employed to assess the biological transformation of 17β-estradiol, with and without BODIPY fluorescent tag.


Emerging contaminants
Method development

Supplementary materials

Supporting Information: BODIPY-labelled estrogens for fluorescence analysis of environmental microbial degradation
MS Word document containing details of the synthesis methods (including verification results for each intermediate), the statistical analyses, and specific results which are summarized in the main text.


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