Biocatalytic cascades towards iminosugar scaffolds reveals promiscuous activity of shikimate dehydrogenases

07 July 2022, Version 1
This content is a preprint and has not undergone peer review at the time of posting.


Protecting group-free chemoenzymatic and biocatalytic cascade synthesis of iminosugars from sugar aminopolyols has been achieved using Galactose oxidase F2 and chemical or enzymatic imine reduction. Putative iminosugar oxidoreductases were identified through genome mining, with several shikimate dehydrogenases (SDH) found to effect iminosugar formation. The SDH catalysed transformation is the first example of such activity on an iminosugar scaffold, and provides support for the reduction of iminosugar intermediates by endogenous enzymes in iminosugar biosynthesis.


shikimate dehydrogenase


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