Industrial potential of the enzymatic synthesis of nucleoside analogs: Existing challenges and perspectives

07 July 2022, Version 2
This content is a preprint and has not undergone peer review at the time of posting.


Nucleoside phosphorylases have progressed from an enzymatic curiosity to a viable synthetic tool. However, despite the recent advances in nucleoside phosphorylase-catalyzed nucleoside synthesis, the widespread application of these enzymes in industrial processes is still lacking. We attribute this gap to three key challenges, which are outlined in this short review. To address these persistent obstacles, we believe that biocatalytic nucleoside synthesis needs to embrace interdisciplinary partnerships with the fields of organic chemistry, process engineering and flow chemistry.


API synthesis
enzyme immobilization
enzyme engineering


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