Crystal Structure and Conformational Dynamics of Pyrococcus furio- sus Prolyl Oligopeptidase

04 July 2022, Version 2
This content is a preprint and has not undergone peer review at the time of posting.


Enzymes in the prolyl oligopeptidase family possess unique structures and substrate specificities that are important for their biological activity and for potential biocatalytic applications. The crystal structures of Pyrococcus furiosus (Pfu) prolyl oligopeptidases (POP) and the corresponding S477C mutant were solved to 1.9 and 2.2 Å resolution, respectively. The wild type enzyme crystallized in an open con- formation, indicating that this state is readily accessible, and it contained bound chloride ions and a prolylproline ligand. These structures were used as starting points for molecular dynamics simulations of Pfu POP conformational dynamics. The simulations showed that large-scale do- main opening and closing occurred spontaneously, providing facile substrate access to the active site. Movement of the loop containing the catalytically essential histidine into a conformation similar to those found in structures with fully-formed catalytic triads also occurred. This movement was modulated by chloride binding, providing a rationale for experimentally observed activation of POP peptidase catalysis by chlo- ride. Thus, the structures and simulations reported in this study, combined with existing biochemical data, provide a number of insights into POP catalysis.


prolyl oligopeptidase
supramolecular catalyst
artificial metalloenzyme
x-ray crystallography
molecular dynamics simulation

Supplementary materials

POP SI 9 4
Complete experimental and computation methods and relevant characterization


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