Physical Chemistry

Lysozyme protected Cu Nano-Cluster: A Photo Switch for the Selective Sensing of Iron (Fe2+)

Authors

Abstract

Protein capped metal nanoclusters gained a lot of recent attention due to their wide range of applications. Here we successfully synthesized a stable, biocompatible lysozyme protected Cu nanocluster (Lys-Cu NC) using an optimized green one-pot protocol under aqueous condition at room temperature. The nanocluster showed a strong photoluminescence intensity (λex = 365 nm, λem = 430 nm) which can be significantly and selectively quenched (off) by Fe2+ ions. Upon addition of NaOH the initial photoluminescence intensity can be recovered completely (on) thereby making the nanocluster a suitable candidate for a photo switch that can be reliably reused for the selective and sensitive detection of Fe2+ ions in the nanomolar (detection limit ~2.5 nM) concentration range. The photoluminescence intensity is also sensitive towards temperature indicating that it can be used as a temperature sensor in different biological systems. It can also be used as an excellent nanoprobe for cell imaging studies.

Content

Thumbnail image of Manuscript.pdf

Supplementary material

Thumbnail image of Supporting Info.docx
SI
Supporting Doc