Electrochemical Recycling of Adenosine Triphosphate in Biocatalytic Reaction Cascades

26 May 2022, Version 1
This content is a preprint and has not undergone peer review at the time of posting.


Adenosine triphosphate (ATP) provides the driving force necessary for critical biological functions in all living organisms. In synthetic biocatalytic reactions, this cofactor is recycled in situ using high-energy stoichiometric reagents, an approach that generates waste and poses challenges with enzyme stability and downstream purification. On the other hand, electrons are a cheap and green source of energy. We report a method that uses electricity to turn over enzymes for ATP generation. The method is simple, robust, and scalable, as well as broadly applicable to complex enzymatic processes including a four-enzyme biocatalytic cascade in the synthesis of the antiviral molnupiravir.


ATP recycling

Supplementary materials

Supporting information
Contains all supplementary materials: Chemicals and Materials, Enzyme Acquisition, Enzyme Purification, Analytical Instrumentation, Electrolysis Equipment, HPLC analytical method, Determination of Conversion and Faradaic Efficiency, UV-Vis Determination of FAD:PO ratio, Electrochemical Studies, Bioelectrochemical stoichiometric ATP formation, Bioelectrochemical glycosylation, Reaction scope, Amino acid and DNA sequences of the enzymes and References.


Comments are not moderated before they are posted, but they can be removed by the site moderators if they are found to be in contravention of our Commenting Policy [opens in a new tab] - please read this policy before you post. Comments should be used for scholarly discussion of the content in question. You can find more information about how to use the commenting feature here [opens in a new tab] .
This site is protected by reCAPTCHA and the Google Privacy Policy [opens in a new tab] and Terms of Service [opens in a new tab] apply.