Electrochemical Recycling of Adenosine Triphosphate in Biocatalytic Reaction Cascades

26 May 2022, Version 1
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

Adenosine triphosphate (ATP) provides the driving force necessary for critical biological functions in all living organisms. In synthetic biocatalytic reactions, this cofactor is recycled in situ using high-energy stoichiometric reagents, an approach that generates waste and poses challenges with enzyme stability and downstream purification. On the other hand, electrons are a cheap and green source of energy. We report a method that uses electricity to turn over enzymes for ATP generation. The method is simple, robust, and scalable, as well as broadly applicable to complex enzymatic processes including a four-enzyme biocatalytic cascade in the synthesis of the antiviral molnupiravir.

Keywords

Biocatalysis
Electrochemistry
ATP recycling
Molnupiravir
Islatravir

Supplementary materials

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Supporting information
Description
Contains all supplementary materials: Chemicals and Materials, Enzyme Acquisition, Enzyme Purification, Analytical Instrumentation, Electrolysis Equipment, HPLC analytical method, Determination of Conversion and Faradaic Efficiency, UV-Vis Determination of FAD:PO ratio, Electrochemical Studies, Bioelectrochemical stoichiometric ATP formation, Bioelectrochemical glycosylation, Reaction scope, Amino acid and DNA sequences of the enzymes and References.
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