Bioactive Fibronectin-III10-DNA Origami Nanofibers Promote Cell Adhesion and Spreading

01 April 2022, Version 1
This content is a preprint and has not undergone peer review at the time of posting.


Integrating proteins with DNA nanotechnology would enable materials with diverse applications in biology, medicine, and engineering. Here, we describe a method for incorporating bioactive fibronectin domain proteins with DNA nanostructures using two orthogonal coiled-coil peptides. One peptide from each coiled-coil pair is attached to a DNA origami cuboid in a multivalent fashion by attaching the peptides to DNA handles. These structures can then be assembled into one-dimensional arrays through the addition of a fibronectin domain linker genetically fused with the complementary peptides to those on the origami. We validate array formation using two different self-assembly protocols and characterize the fibers by atomic force and electron microscopy. Finally, we demonstrate that surfaces coated with the protein-DNA nanofibers can serve as biomaterial substrates for fibroblast adhesion and spreading, with the nanofibers enhancing bioactivity compared with the monomeric protein.


DNA origami
Cell-material interface
Extracellular matrix
DNA nanotechnology


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