Working Paper
Authors
- Alex Buchberger Arizona State University ,
- Kyle Riker University of North Carolina at Chapel Hill ,
- Julio Bernal-Chanchavac Arizona State University ,
- Raghu Pradeep Narayanan Arizona State University ,
- Chad R. Simmons Arizona State University ,
- Nour Eddine Fahmi Arizona State University ,
- Ronit Freeman
University of North Carolina at Chapel Hill ,
- Nicholas Stephanopoulos Arizona State University
Abstract
Integrating proteins with DNA nanotechnology would enable materials with diverse applications in biology, medicine, and engineering. Here, we describe a method for incorporating bioactive fibronectin domain proteins with DNA nanostructures using two orthogonal coiled-coil peptides. One peptide from each coiled-coil pair is attached to a DNA origami cuboid in a multivalent fashion by attaching the peptides to DNA handles. These structures can then be assembled into one-dimensional arrays through the addition of a fibronectin domain linker genetically fused with the complementary peptides to those on the origami. We validate array formation using two different self-assembly protocols and characterize the fibers by atomic force and electron microscopy. Finally, we demonstrate that surfaces coated with the protein-DNA nanofibers can serve as biomaterial substrates for fibroblast adhesion and spreading, with the nanofibers enhancing bioactivity compared with the monomeric protein.
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