Oligodeoxynucleotides (ODNs) are typically purified and analysed with HPLC equipped with a UV-Vis detector. Quantities of ODNs are usually determined using a UV-Vis spectrometer separately after HPLC, and are reported as optical density at 260 nm (OD260). Here, we describe a method for direct determination of OD260 of ODNs using the area of the peaks in HPLC profiles.
Determination of Optical Density (OD) of Oligodeoxynucleotide from HPLC Peak Area
15 March 2022, Version 1
This content is a preprint and has not undergone peer review at the time of posting.