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Determination of Optical Density (OD) of Oligodeoxynucleotide from HPLC Peak Area

15 March 2022, Version 1

Abstract

Oligodeoxynucleotides (ODNs) are typically purified and analysed with HPLC equipped with a UV-Vis detector. Quantities of ODNs are usually determined using a UV-Vis spectrometer separately after HPLC, and are reported as optical density at 260 nm (OD260). Here, we describe a method for direct determination of OD260 of ODNs using the area of the peaks in HPLC profiles.

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Now Published

Determination of optical density (OD) of oligodeoxynucleotide from HPLC peak area [opens in a new tab]
Komal Chillar, Yipeng Yin, Adikari M. Dhananjani N. Eriyagama, Shiyue Fang journal article
PeerJ Analytical Chemistry , Volume 4
Online publication date: Jul 07, 2022

Version History

Mar 15, 2022 Version 1

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License

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The content is available under CC BY 4.0 License CreativeCommons.org [opens in a new tab]

DOI

10.26434/chemrxiv-2022-hdqzr
D O I: 10.26434/chemrxiv-2022-hdqzr [opens in a new tab]

Funding

NSF
1954041
NIH
GM109288

Author’s competing interest statement

The author(s) have declared they have no conflict of interest with regard to this content

Ethics

The author(s) have declared ethics committee/IRB approval is not relevant to this content

Keywords

HPLC
UV
OD
oligonucleotide
DNA
RNA
optical density
quantification

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Publication

This content is an early or alternative research output and has not been peer-reviewed at the time of posting.