Hydride state accumulation in native [FeFe]-hydrogenase with the physiological reductant H2 supports its catalytic relevance

25 February 2022, Version 1
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

Small molecules in solution may affect the stability of catalytic states and produce off-cycle states that can be mistaken for catalytically relevant species. Here we show that the hydride state (Hhyd), a proposed central intermediate in the catalytic cycle of [FeFe] hydrogenase, can be formed in wild-type [FeFe]-hydrogenases treated with H2 in strict absence of other, non-biological, reductants. Moreover, we reveal a new state with unclear role in catalysis induced by common low pH buffers.

Keywords

catalysis
hydrogenases

Supplementary materials

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Title
Supporting Information: Hydride state accumulation in native [FeFe]-hydrogenase with the physiological reductant H2 supports its catalytic relevance
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Supporting information
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