Dynamic Multicomponent Reactions Directed Self-assembled G-quadruplex Inherent Antibacterial Hydrogel

Now-a-days inherent antibacterial hydrogels have gained significant attention due to their utilization against infectious bacteria. Herein, we focus on the development of injectable, self-healable, dynamic, G-quadruplex hydrogel with inherent antibacterial activity. The dynamic self-assembled hydrogel is constructed upon multicomponent reactions (MCR) among guanosine, 2-formylphenylboronic acid and amino acid/peptides in presence of potassium ions. The role of amino acid/peptides toward the formation of G-quadruplex hydrogel is studied in details. G-quadruplex structure is formed via the π-π stacking of G-quartets. The formation of G-quadruplex is investigated by thioflavin T binding assay, CD spectroscopy and PXRD. The formation of the dynamic imino-boronate bond in the hydrogels is well characterised by temperature dependent 11B NMR (VT-NMR) and FT-IR spectroscopy. Furthermore HR-TEM images and rheological experiments reveal the fibrillar networks and the viscoelastic property of the hydrogels. The presence of the dynamic imino-boronate ester bonds makes the hydrogel injectable and self-healable in nature. These dynamic G-quadruplex hydrogels show potential antibacterial activity against a series of Gram positive and Gram negative bacteria. Such injectable and self-healable hydrogels could be used in various applications in the field of biomedical science.