BTDAzo - a photoswitchable TRPC5 channel activator

28 January 2022, Version 1
This content is a preprint and has not undergone peer review at the time of posting.


Photoswitchable reagents to modulate protein activity are powerful tools for high-spatiotemporal-precision control over endogenous biological functions. TRPC5 is a Ca2+-permeable cation channel with distinct tissue-specific roles, ranging from synaptic function to hormone regulation. Achieving spatially-resolved control over TRPC5 activity in particular cells or tissues, and temporal regulation in targeted cells, are therefore crucial milestones towards understanding and harnessing the biology of TRPC5. Here we develop the first photoswitchable TRPC5-modulating reagent, BTDAzo, towards reaching this goal. BTDAzo can photocontrol TRPC5 currents in cell culture, as well as controlling endogenous TRPC5-based neuronal Ca2+ responses in mouse brain slices. BTDAzos are also the first reported azo-benzothiadiazines, an accessible and conveniently derivatised azoheteroarene that features excellent two-colour photoswitching. BTDAzo's TRPC5 control across relevant channel biology settings makes it appropriate for a range of dynamically reversible photoswitching studies in TRP channel biology, aiming to decipher the various biological roles of this centrally important ion channel.


optical control
cation channel currents
TRP channel
ion channel
transient receptor potential
TRP ligand
TRP activator

Supplementary materials

Supporting Information for: BTDAzo - a photoswitchable TRPC5 channel activator
Supporting Information includes Synthetic procedures, photocharacterization, biological methods and analytical data

Supplementary weblinks


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