Working Paper
Authors
- Manxi Yang
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Purdue University West Lafayette
, - Hang Hu Purdue University West Lafayette ,
- Pei Su Northwestern University & Purdue University West Lafayette ,
- Paul M. Thomas Northwestern University ,
- Jeannie M. Camarillo Northwestern University ,
- Joseph B. Greer Northwestern University ,
- Bryan P. Early Northwestern University ,
- Ryan T. Fellers Northwestern University ,
- Neil L. Kelleher Northwestern University ,
- Julia Laskin Purdue University West Lafayette
Abstract
Unraveling the complexity of biological systems relies on the development of new approaches for spatially resolved proteoform-specific analysis of the proteome. Herein, we employ nanospray desorption electrospray ionization mass spectrometry imaging (nano-DESI MSI) for the proteoform-selective imaging of biological tissues. Nano-DESI generates multiply charged protein ions, which is advantageous for their structural characterization using tandem mass spectrometry (MS/MS) directly on the tissue. Proof-of-concept experiments demonstrate that nano-DESI MSI combined with on-tissue top-down proteomics is ideally suited for the proteoform-selective imaging of tissue sections. Using rat brain tissue as a model system, we provide the first evidence of differential proteoform expression in different regions of the brain.
Version notes
The manuscript was polished and proteoform assignments were updated.
Content
Supplementary material
Supporting information
This document contains the descriptions of methods and experiments, and supplemental figures.
Supporting table
This document contains the proteoforms assignments.
