Analytical Chemistry

A Versatile AIE Fluorogen with Selective Reactivity to Primary Amines for Monitoring Amination, Protein Labeling and Mitochondrial Staining

Authors

  • Xinyuan He Hong Kong University of Science and Technology ,
  • Huilin Xie Hong Kong University of Science and Technology ,
  • Lianrui Hu Hong Kong University of Science and Technology ,
  • Pengchao Liu City University of Hong Kong ,
  • Changhuo Xu Hong Kong University of Science and Technology ,
  • Wei He Hong Kong University of Science and Technology ,
  • Wutong Du Hong Kong University of Science and Technology ,
  • Siwei Zhang Hong Kong University of Science and Technology ,
  • Hao Xing Hong Kong University of Science and Technology ,
  • Xinyue Liu Hong Kong University of Science and Technology ,
  • Hojeong Park Hong Kong University of Science and Technology ,
  • Tsz Shing Cheung Hong Kong University of Science and Technology ,
  • Min-Hui Li PSL Research University ,
  • Ryan T. K. Kwok Hong Kong University of Science and Technology ,
  • Jacky W. Y. Lam Hong Kong University of Science and Technology ,
  • Jian Lu City University of Hong Kong ,
  • Ben Zhong Tang Hong Kong University of Science and Technology

Abstract

Specific bioconjugation for native primary amines is highly valuable for both chemistry and biomedical research. Despite all the efforts, scientists lack a proper strategy to achieve high selectivity for primary amines, not to mention the requirement of fast response for real applications. Herein, in this work, we report a chromone-based aggregation-induced emission (AIE) fluorogen called CMVMN as a self-reporting bioconjugation reagent for selective primary amine identification, and its applications for monitoring bioprocesses of amination and protein labeling. CMVMN is AIE-active and is capable of solid-state sensing. Thus, its electrospun films are manufactured for visualization of amine diffusion and leakage process. CMVMN also shows good biocompatibility and potential mitochondria-staining ability, which provides new insight for organelle-staining probe design. Combined with its facile synthesis and good reversibility, CMVMN not only shows wide potential applications in biology, but also offers new possibilities for molecular engineering.

Content

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