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An expanded palette of fluorogenic HaloTag probes with enhanced contrast for targeted cellular imaging

19 November 2021, Version 1
Working Paper
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

We report on new fluorogenic HaloTag probes based on a molecular rotor design. Thanks to their viscosity-sensitive emission, the probes light-up upon reaction with the protein self-labeling tag HaloTag. The palette of probes cover an emission range from green to red and exhibit remarkably low non-specific signal that enabled wash-free targeted imaging of intracellular organelles and proteins with good contrast in live Hela cells.

Keywords

Fluorogenic probes
HaloTag
Subcellular targeting
Fluorescence imaging

Supplementary materials

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Supplementary Information
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materials and methods, synthetic and molecular biology procedures, additional figures and schemes
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Version History

Nov 19, 2021 Version 1

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The content is available under CC BY NC ND 4.0 [opens in a new tab]

DOI

10.26434/chemrxiv-2021-ttc5g
D O I: 10.26434/chemrxiv-2021-ttc5g [opens in a new tab]

Funding

Agence Nationale de la Recherche
ANR-18-CE44-0006
European Research Council
ERC-2016-CoG-724705 FLUOSWITCH

Author’s competing interest statement

The author(s) have declared they have no conflict of interest with regard to this content

Ethics

The author(s) have declared ethics committee/IRB approval is not relevant to this content

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