Polymerase-mediated synthesis of artificial RNA-DNA metal base pairs

18 November 2021, Version 1
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

Metal-mediated base pairs are formed by the connection of two nucleobases via coordination to a metal cation. The resulting metal-containing duplexes have been used in a large variety of applications ranging from allosteric control of functional nucleic acids to the construction of nanowires. Recently, enzymatic approaches are being developed for the construction of metal-mediated base pairs. Here, we have studied the possibility of constructing HgII- and AgI-mediated DNA/RNA hetero base pairs using primer extension reactions. The high kinetic and thermodynamic stabilities of metal base pairs can be harnessed to trigger the formation of multiple rU-HgII-dT base pairs.

Keywords

metal base pairs
DNA
RNA
polymerases

Supplementary materials

Title
Description
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Title
ESI for Polymerase-mediated synthesis of artificial RNA-DNA metal base pairs
Description
Experimental protocols, additional gel images, and melting curves
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Supplementary weblinks

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