User-friendly, magnetically sealed plug-and-play sensor module for online electrochemical sensing for fluidic devices

The growth in fluidic devices, such as organ-on-chip (OOC) technology, comes with a need for growth in sensing capabilities of key biomolecules to help elucidate changes during the time course of experiments. We developed an on-line, easy-to-assemble, 3D-printed electrochemical sensor module that is magnetically sealed for ease of assembly. The sensor module includes a plug-and-play format for electrochemical sensors made in finger-tight fittings to allow for a wide selection of experimental set-ups and target molecules. Here, we report the feasibility of the sensor module as well as demonstrate its use for electrochemical sensing with integrated thermoplastic electrodes (TPEs). The sensor module withstood over 300 kPa of backpressure and demonstrated reliable performance with TPEs when using cyclic voltammetry (CV) and amperometry under flow conditions. CVs using the ferri/ferrocyanide (K3/4[Fe(CN)6]) redox system demonstrate that the sensor module does not hinder the expected linear response with respect to analyte concentration. Further CVs and amperometry demonstrated the use of the sensor module under flow conditions. Such success in device design and usability is promising for future work using the on-line sensor module with a variety of applications.