Freeze-Float System for High-throughput Measurement of Ice Nucleation

In this publication, we developed the high throughput screening implementation of freeze-float selection platform system we established in the previous publication. The goal of this publication is to expand the system to higher throughput and accuracy. In the following sections, we describe the steps for automated droplets generations, adaptation of freeze-float selection using controlled and uniform temperature cooling system, and semi-automated droplets detection program. We aimed to improve previously published system to add functional advantages, such as; a) increased efficiency of the screening with fewer manipulation steps; b) increased accuracy of measurement due to the increased sample size; c) increased uniformity of temperature distribution by incorporating the controlled-rate freezer. In the following sections, we describe the steps for automated droplets generations, freeze-float system adaptations using controlled and uniform temperature cooling system, and semi-automated droplets detection program.