Wide-field Surface-Enhanced Coherent Anti-Stokes Raman Scattering Microscopy

21 October 2021, Version 1
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

Surface-enhanced Raman scattering (SERS) spectroscopy has been used extensively to study biology, chemistry, and materials. However, a point-by-point SERS mapping is time-consuming, taking minutes to hours for large-scale imaging. Here, we report a wide-field surface-enhanced coherent anti-Stokes Raman scattering (WISE-CARS) microscopy for monitoring nanotags in live cells and label-free detection of metabolic molecules. The WISE-CARS microscope achieves an imaging speed as fast as 120 frames per second for a large field of view of 130 microns X 130 microns. By spectral focusing of femtosecond lasers, a hyperspectral WISE-CARS stack of 120 frames can be acquired with a spectral resolution of 10 cm-1, where over 1 million Raman spectra are parallelly recorded within 0.5 seconds. As applications, we demonstrate time-lapse, 3D WISE-CARS imaging of nanotags in live cells as well as label-free detection of adenine released from S. aureus.

Keywords

wide-field microscopy
surface-enhanced coherent anti-stokes Raman scattering
3D cell imaging
bacteria detection

Supplementary materials

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Video 1. Hyperspectral WISE-CARS imaging of Au NPs substrate.
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A hyperspectral SECARS imaging cube of Mpy adsorbed on aggregated Au NPs substrate
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Video 3. Hyperspectral WISE-CARS imaging of live cells.
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The hyperspectral SECARS spectra of Mpy nanotags in live cells.
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Video 2. Movements of nanotags in live cells
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60-frame video recorded to show the movements of nanotags inside a live cell during 1 s period
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Video 4. 3D WISE-CARS imaging of live cells.
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Recorded bright views of T24 cells at different focus planes.
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Video 5. 3D WISE-CARS imaging of live cells.
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SECARS imaging of Mpy nanotags distributed in T24 cells at different focus planes.
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Video 6. 3D projection of nanotags in live cells.
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The 3D projection shows the 3D distributions of nanotags in live cells.
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