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In vitro characterization of a nitro-forming oxygenase involved in 3-(trans-2’-aminocyclopropyl)alanine biosynthesis

15 October 2021, Version 1
Working Paper
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

In vitro characterization experiments revealed the formations of 3-(trans-2’-aminocyclopropyl)alanine ((3-Acp)Ala) and 3-(trans-2’-nitrocyclopropyl)alanine ((3-Ncp)Ala) are originated via two homologous proteins BelK and HrmI, which regioselectively catalyze Nε-nitration of L-lysine. The two enzymes belong to the emerging heme-oxygenase-like diiron oxidase and oxygenase (HDO) superfamily and the catalytic center of BelK is validated by homology modeling and site-directed mutations. Based on the in vitro characterization, the biosynthetic pathways of (3-Acp)Ala and (3-Ncp)Ala are first proposed.

Keywords

Nitro-forming oxygenase
HDO superfamily protein
Belactosin A
Hormaomycin
Cyclopropyl ring

Supplementary materials

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Description
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Supporting Information
Description
Full experimental details, in silico analysis, SDS-PAGE, homology modeling, MS data, NMR spectra and sequence alignments.
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Now Published

In vitro characterization of a nitro-forming oxygenase involved in 3-(trans-2’-aminocyclopropyl)alanine biosynthesis [opens in a new tab]
Linlin Pang, Weijing Niu, Yuwei Duan, Liujie Huo, Aiying Li, Jiequn Wu, Youming Zhang, Xiaoying Bian, Guannan Zhong journal article
Engineering Microbiology , Volume 2, Issue 1
Print publication date: Mar, 2022

Version History

Oct 15, 2021 Version 1

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The content is available under CC BY NC ND 4.0 [opens in a new tab]

DOI

10.26434/chemrxiv-2021-zx6rx
D O I: 10.26434/chemrxiv-2021-zx6rx [opens in a new tab]

Funding

National Key R&D Program of China
2019YFA0905700
National Natural Science Foundation of China
21907057, 32070060
Shandong Provincial Natural Science Foundation, China
ZR2019JQ11, ZR2019ZD18
Natural Science Foundation of Jiangsu Province, China
BK20190201
the 111 project of Shandong University, China
B16030
the Youth Interdisciplinary Innovative Research Group of Shandong University, China
2020QNQT009
the Future Plan for Young Scholars of Shandong University, China
the Fundamental Research Funds of Shandong University, China
2019GN032

Author’s competing interest statement

The author(s) have declared they have no conflict of interest with regard to this content

Ethics

The author(s) declare that they have sought and gained approval from the relevant ethics committee/IRB for this research and its publication.

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