Tuning dynamic DNA- and peptide-driven self-assembly in DNA-peptide conjugates

06 October 2021, Version 1
This content is a preprint and has not undergone peer review at the time of posting.


DNA-peptide conjugates offer an opportunity to marry the benefits of both biomolecules, such as the high level of control and programmability found with DNA and the chemical diversity and biological stability of peptides. These hybrid systems offer great potential in fields such as therapeutics, nanotechnology, and robotics to name a few. Using the first DNA-β-turn peptide conjugate, we present three studies designed to investigate the self-assembly of DNA-peptide conjugates over a period of 28 days. Time-course studies, such as these have not been previously conducted for DNA-peptide conjugates, although they are common in pure peptide assembly, for example in amyloid research. By using aging studies to assess the structures produced, we gain insights into the dynamic nature of these systems. The first study explores the influence varying amounts of DNA-peptide conjugates have on the self-assembly of our parent peptide. Study 2 explores how DNA and peptide can work together to change the structures observed during aging. Study 3 investigates the presence of orthogonality within our system by switching the DNA and peptide control on and off independently. These results show that two orthogonal self-assemblies can be combined and operated either independently or in tandem within a single macromolecule, with both spatial and temporal effects upon the resultant nanostructures.


DNA nanotechnology
Peptide nanotechnology
Peptide-oligonucleotide conjugates
Supramolecular chemistry

Supplementary materials

Supplementary Information for DNA-ILVAGK manuscript
Synthetic and procedural details, molecular characterisation, further DLS, AFM, and CD data.


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