Physical Chemistry

Profiling single-molecule reaction kinetics under nanopore confinement

Authors

  • Wei Liu State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University ,
  • Zhonglin Yang State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University ,
  • Chaonan Yang State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University ,
  • Yi-Lun Ying State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, P.R. China. ; Chemistry and Biomedicine Innovation Center, Nanjing University, P.R. China ,
  • Yi-Tao Long State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University

Abstract

The development of single-molecule reaction inside nanoconfinement is benefit to study the intrinsic molecular mechanism of a complex chemical reaction. However, the reaction kinetics model of single-molecule reaction inside confinement remains elusive. Herein we engineered the Aerolysin nanopore reactor to elaborate the single-molecule reaction kinetics inside nanoconfinement. By identifying bond forming and non-forming events directly, a four-state kinetics model is proposed for the first time. Our results demonstrated that the single-molecule reaction kinetics inside a nanopore depends on the voltage-dependent frequency of captured individual reactant and the fraction of effective collision inside nanopore confined space. This new insight will guide the design of nanoconfinement for resolving the single-molecule chemistry, and shed light on the mechanistic understanding of dynamic covalent chemistry in-side a nanopore

Content

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Supplementary material

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Supplementary Information
1. Materials and methods 2. Supplementary results and discussions 3. References