Working Paper
Authors
- Tanaya Manna Centre of Biomedical Research-India ,
- Anshul Rajput Centre of Biomedical Research-India ,
- Nirmal Saha Centre of Biomedical Research-India ,
- Amit Mondal Centre of Biomedical Research -India ,
- Syed Masood Husain
Centre of Biomedical Research-India
Abstract
The first asymmetric total synthesis of fungal secondary metabolites, (R)-nodulone C (4) and trans-nodulone D (5) has been reported through the chemoenzymatic approach. The strategy utilizes NADPH-dependent naphthol reductases of Magnaporthe grisea for the reduction of putative biosynthetic substrates, synthesized non-enzymatically in multiple steps. A dihydronaphthalenone 32 and cis-nodulone D (30) has also been synthesized chemoenzymatically. The work implies for similar steps during the biosynthesis of nodulones and their analogs with the involvement of tetrahydroxynaphthalene reductase related enzyme(s).
Content

Supplementary material

Supplementary Information
The file contains procedure for enzyme expression and synthesis of substrates along with NMR, mass and CD spectral data.