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A selenium-based cysteine surrogate for protein chemical synthesis

16 September 2021, Version 1
Working Paper
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

We provide in this protocol detailed procedures for the synthesis of SetCys cysteine surrogate and its use for chemical synthesis of proteins through the redox-controlled assembly of three peptide segments in one-pot.

Keywords

chemical protein synthesis
cysteine surrogate
selenium
native chemical ligation
NCL
bis(2-sulfanylethyl)amido
SEA-mediated ligation
hepatocyte growth factor
Kringle domain
one-pot
chemoselective
SetCys
redox
chemical ligation

Supplementary materials

Title
Description
Actions
Title
NMR data
Description
This compressed folder contains raw NMR data (FID files) for key selenated compounds, for comparison.
Actions

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Version History

Sep 16, 2021 Version 1

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The content is available under CC BY NC ND 4.0 [opens in a new tab]

DOI

10.26434/chemrxiv-2021-5nn16
D O I: 10.26434/chemrxiv-2021-5nn16 [opens in a new tab]

Author’s competing interest statement

The author(s) have declared they have no conflict of interest with regard to this content

Ethics

The author(s) declare that they have sought and gained approval from the relevant ethics committee/IRB for this research and its publication.

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