Nanoscience

Odorant Binding Changes the Electrical Properties of Olfactory Receptors at the Nanoscale

Authors

  • Anna Lagunas Biomedical Research Networking Center in Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN); Institute for Bioengineering of Catalonia (IBEC), Barcelona Institute of Science and Technology ,
  • Christine Belloir Centre des Sciences du Goût et de l’Alimentation, AgroSup Dijon, CNRS, INRAE, Université Bourgogne Franche-Comté ,
  • Loic Briand Centre des Sciences du Goût et de l’Alimentation, AgroSup Dijon, CNRS, INRAE, Université Bourgogne Franche-Comté ,
  • Pau Gorostiza Institute for Bioengineering of Catalonia (IBEC), Barcelona Institute of Science and Technology; Biomedical Research Networking Center in Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN); Catalan Institution for Research and Advanced Studies (ICREA) ,
  • Josep Samitier Institute for Bioengineering of Catalonia (IBEC), Barcelona Institute of Science and Technology; Biomedical Research Networking Center in Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN); Department of Electronics and Biomedical Engineering, University of Barcelona (UB)

Abstract

The transduction of odorant binding into cellular signaling by olfactory receptors (ORs) is not understood and knowing its mechanism would enable developing new pharmacology and biohybrid electronic detectors of volatile organic com-pounds bearing high sensitivity and selectivity. The electrical characterization of ORs in bulk experiments is subject to microscopic models and assumptions. We have directly determined the nanoscale electrical properties of ORs immobilized in a fixed orientation, and their change upon odorant binding, using electrochemical scanning tunneling microscopy (EC-STM) in near-physiological conditions. Recordings of current versus time, distance, and electrochemical potential allows determining the OR impedance parameters and their dependence with odorant binding. Our results allow validating OR structural-electrostatic models and their functional activation processes.

Content

Thumbnail image of Lagunasetal2021_OR.pdf

Supplementary material

Thumbnail image of Lagunasetal2021_SI_OR.docx
Supplementary Information for Odorant Binding Changes the Electrical Properties of Olfactory Receptors at the Nanoscale
The Supplementary Information file includes a materials section, sample preparation description, details on electrochemical scanning tunneling microscopy (EC-STM) measurements, statistics, atomic force microscopy measurements, conductance fitting, current-distance (I-z) and current-time measurements (I-t), and open-circuit voltage (VOC) analysis.