Detection of single amino acid differences in haemoglobin from blood samples using a nanopore

09 July 2021, Version 1
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

The real-time identification of protein biomarkers is under intense investigation for the development of point-of-care and portable devices. Here we use a PlyAB biological nanopore to detect a range of folded Haemoglobin (Hb) variants in blood samples. Blockades of HbA and sickle cell anaemia HbS, which differ by just one amino acid, and fetal HbF were distinguished with more than 97% accuracy on the basis of individual events. This nanopore approach is quick and straightforward. It can count and confirm the identity of Hb variants instantaneously directly from blood samples. Therefore, PlyAB nanopores are promising single-molecule nanoreactors for real-time folded protein analysis, and identification with immediate application in point-of-care Hb counting and sickle cell anaemia diagnosis.

Keywords

nanopore
single-molecule
blood
detection

Comments

Comments are not moderated before they are posted, but they can be removed by the site moderators if they are found to be in contravention of our Commenting Policy [opens in a new tab] - please read this policy before you post. Comments should be used for scholarly discussion of the content in question. You can find more information about how to use the commenting feature here [opens in a new tab] .
This site is protected by reCAPTCHA and the Google Privacy Policy [opens in a new tab] and Terms of Service [opens in a new tab] apply.