Influence of divalent cations in the protein crystallization process assisted by Lanthanide-based additives.

18 June 2021, Version 1


The use of lanthanide complexes as powerful auxiliaries for biocrystallography prompted us to systematically analyze the influence of the commercial crystallization kit composition on the efficiency of two lanthanide additives: [Eu(DPA)3]3- and Tb-Xo4. This study reveals that the tris-dipicolinate complex presents a lower chemical stability and a strong tendency to false positives which are detrimental for its use in high-throughput robotized crystallization platform. On the other hand, the Tb-Xo4 is perfectly soluble in the crystallization media, stable in the presence of alkaline-earth dications and slowly decomposed (within days) by trans-metalation with transition metals. This article also highlight a potential synergy of interactions between the Tb-Xo4 and components of the crystallization mixtures leading to the formation of complex adducts like {AdkA/Tb-Xo4/Mg2+/glycerol} in the protein binding sites.


protein crystallography


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