Coding Synthetic Chemistry Strategies into Bacterial Designer Cells

11 May 2021, Version 1
This content is a preprint and has not undergone peer review at the time of posting.


Following a synthetic chemistry blueprint for the valorization of lignocellulosic platform chemicals, this study showcases a so far unprecedented approach to implement non-natural enzyme modules in vivo. For the design of a novel functional whole cell tool, two purely abiotic transformations were incorporated into a recombinant bacterial host that allows production of complex lactone building blocks. This whole cell system streamlines the synthetic cascade, eliminates isolation and purification steps, and provides a high degree stereoselectivity that has so far been elusive in the chemical methodology.


artificial enzyme modules
platform chemicals
whole cell biotransformations

Supplementary materials

2021 Deska DesignerCell ESI


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