Towards Reproducible Enzyme Modeling with Isothermal Titration Calorimetry

10 May 2021, Version 1
This content is a preprint and has not undergone peer review at the time of posting.


An experimental workflow to provide detailed information of the molecular mechanisms of enzymes is described. This workflow will help in the application of enzymes in technical processes by providing crucial parameters needed to plan, model and implement biocatalytic processes more efficiently. These parameters are homogeneity of the enzyme sample (HES), kinetic and thermodynamic parameters of enzyme kinetics and binding of reactants to enzymes. The techniques used to measure these properties are dynamic light scattering (DLS), UV-Vis spectrophotometry and isothermal titration calorimetry (ITC) respectively. The workflow is standardized by the use of SOPs and python-scripted data analysis.

We have used the NADPH-dependent alcohol dehydrogenase Gre2p as a challenging enzyme to demonstrate the power of this workflow. Our work highlights the utility for combined binding and kinetic studies for such complex multi-substrate reactions and the importance of sample quality control during experiments.


(S)-stereoselective ketoreductase
kinetic model
quality control
dynamic light scattering
UV-Vis spectrophotometry

Supplementary materials

Ott Rabe Niemeyer GYGLI 2021 SI

Supplementary weblinks


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