Flavin-Tag: A Facile Method for Site-Specific Labeling of Proteins with a Flavin Fluorophore

23 April 2021, Version 1
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

Flavin-tag: we developed a facile method to label a target protein with a flavin. By using a short peptide (Flavin-tag), recognized by a flavinylating enzyme, FMN can be covalently incorporated. Attachment of the flavin chromo- and fluorophore can be performed in vivo and in vitro rendering the Flavin-tag a flexible tool for protein labeling.

Keywords

protein labelling
bioconjugating enzymes
FMN
flavin
fluorophore
chromophore

Supplementary materials

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Flavin-tag SI
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