Time-Dependent Influence of High Glucose Environment on the Metabolism of Neuronal Immortalized Cells

16 March 2021, Version 1
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

In this work the effect of glucose concentration on the metabolome of living hippocampal HN9.10e neurons was studied. This cell line represents a reliable, in vitro model of one of the most vulnerable regions of central nervous system. Targeted metabolites were analyzed in the cell culture medium by two direct methods, namely liquid chromatography – diode array detection and headspace – solid phase micro extraction – gas chromatography – mass spectrometry. Twenty-two metabolites were simultaneously identified and quantified in the growth medium of the cells, treated with 25, 50 or 75 mM glucose, sampled along 8 days to mimic a prolonged hyperglycemia. The results of statistical analysis showed the clear impairment of neuronal metabolism already after 48 hours, represented by a significant reduction of the metabolic activity.

Keywords

Targeted metabolomics
hyperglycemia
primary cultured hippocampal neurons
HPLC-DAD
cell death
3-way PCA

Supplementary materials

Title
Description
Actions
Title
Supplementary Table 5
Description
Actions
Title
Supplementary Table 4
Description
Actions
Title
Supplementary Table 3
Description
Actions
Title
Supplementary Table 2
Description
Actions
Title
Supplementary Table 1
Description
Actions

Comments

Comments are not moderated before they are posted, but they can be removed by the site moderators if they are found to be in contravention of our Commenting Policy [opens in a new tab] - please read this policy before you post. Comments should be used for scholarly discussion of the content in question. You can find more information about how to use the commenting feature here [opens in a new tab] .
This site is protected by reCAPTCHA and the Google Privacy Policy [opens in a new tab] and Terms of Service [opens in a new tab] apply.