Analytical Chemistry

Time-Dependent Influence of High Glucose Environment on the Metabolism of Neuronal Immortalized Cells

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Abstract

In this work the effect of glucose concentration on the metabolome of living hippocampal HN9.10e neurons was studied. This cell line represents a reliable, in vitro model of one of the most vulnerable regions of central nervous system. Targeted metabolites were analyzed in the cell culture medium by two direct methods, namely liquid chromatography – diode array detection and headspace – solid phase micro extraction – gas chromatography – mass spectrometry. Twenty-two metabolites were simultaneously identified and quantified in the growth medium of the cells, treated with 25, 50 or 75 mM glucose, sampled along 8 days to mimic a prolonged hyperglycemia. The results of statistical analysis showed the clear impairment of neuronal metabolism already after 48 hours, represented by a significant reduction of the metabolic activity.

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Supplementary material

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Supplementary Table 5
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Supplementary Table 2
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Supplementary Table 1