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Detection of Metabolite-Protein Interactions in Complex Biological Samples by High-Resolution Relaxometry: Towards Interactomics by NMR

01 February 2021, Version 2

Abstract

In this manuscript, we introduce an NMR method to investigate the interaction of metabolites and other small molecules with proteins in biological fluids. Our approach, based on high-resolution relaxometry identifies the interaction of small molecules with proteins in blood serum and is used for the quantitative analysis of binding competition in situ.

Keywords

metabolomics
NMR
relaxometry
interactomics
high-resolution relaxometry

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Version History

Feb 01, 2021 Version 2

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The content is available under CC BY NC ND 4.0 [opens in a new tab]

DOI

10.26434/chemrxiv.13603301.v2
D O I: 10.26434/chemrxiv.13603301.v2 [opens in a new tab]

Funding

European Research Council (ERC) Grant agreement 825404 (RELAX-MAX)
FET-Open Grant agreement 899683 (HIRES-MULTIDYN)
EURELAX COST Action CA15209
AIRC fellowship (Italy)
Fondazione Cassa di Risparmio di Firenze

Author’s competing interest statement

J.-M. T. and T. M. are employees of Bruker Biospin.

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