Nanoscience

Local, Single-Molecule Oxidative Cleaving of DNA Origami by C60 on an AFM Tip

Abstract

Spatially controlled single-molecule oxidation of DNA was performed by photocatalytic generation of singlet oxygen on chemically functionalized AFM tips. A waffle-type DNA origami deposited on a mica surface is site-specifically destroyed by generation of reactive oxygen species at the AFM tip, which was modified with C60-tripod photocatalyst. Upon AFM scanning under photoirradiation, DNA morphology changes, corresponding to oxidative damage were clearly observed at the single-molecule level. The DNA cleavage occurred with strict dependence on photoirradiation and the presence of C60 on the AFM tip.

Content

Thumbnail image of Main Txt_new.pdf

Supplementary material

Thumbnail image of supporting_new.pdf
supporting new