Local, Single-Molecule Oxidative Cleaving of DNA Origami by C60 on an AFM Tip

03 February 2021, Version 1
This content is a preprint and has not undergone peer review at the time of posting.


Spatially controlled single-molecule oxidation of DNA was performed by photocatalytic generation of singlet oxygen on chemically functionalized AFM tips. A waffle-type DNA origami deposited on a mica surface is site-specifically destroyed by generation of reactive oxygen species at the AFM tip, which was modified with C60-tripod photocatalyst. Upon AFM scanning under photoirradiation, DNA morphology changes, corresponding to oxidative damage were clearly observed at the single-molecule level. The DNA cleavage occurred with strict dependence on photoirradiation and the presence of C60 on the AFM tip.


DNA origami
Fullerene C60
Reactive oxygen species
Singlet oxygen
Atomic force microscopy
PeakForce tapping

Supplementary materials

supporting new


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