Using tailored nanodiamonds with negatively charged silicon-vacancy (SiV-) color centers we performed a series of intracellular experiments. These nanodiamonds with SiV- centers (NDs-SiV-) were obtained by high-pressure high-temperature (HPHT) synthesis based on metal catalysts-free hydrocarbon HPHT growth. They were coated by a polypeptide biopolymer allowing efficient cellular uptake. High photostability and narrow emission in the near infrared region allows tracking of functionalized NDs-SiV- in living cells. The excellent optical properties render NDs-SiV- promising nanoemitters for various bioimaging applications. Furthermore, cellular tracking with detailed time, spatial, and spectral information paves the way to intracellular thermometry based on NDs-SiV-.
Liu 2021 SI