Molecular-Level Interactions of Nanodisc-Forming Copolymers Dissected by EPR Spectroscopy

25 January 2021, Version 1
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

In this study, we focus on analyzing the non-covalent interaction patterns between three lipid-nanodisc-forming polymers and nitroxide radicals which are used as small organic tracer molecules. Besides the negatively charged polymers DIBMA and SMA(2:1), we characterize the solvation behavior of a new zwitterionic styrene/maleic amide sulfobetaine copolymer named SMA-SB. The used nitroxide spin probes vary in their respective chemical structure, allowing the report of different local micropolarities and nanoscopic regions by recording temperature-dependent continuous-wave electron paramagnetic resonance (CW EPR) spectra. In combination with dynamic light scattering (DLS) and electrophoretic light scattering (ELS) experiments, we are able to provide a nanoscopic interpretation of the dominant interaction patterns between the polymer and the chosen guest molecule.

Keywords

Nanodiscs
EPR Spectroscopy
DLS
ELS
UCST
Polymer
Polyemectrolytes
SMA Polymers
DIBMA Polymers

Supplementary materials

Title
Description
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Title
ND Polymer Macromolecules SI
Description
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