Analytical Chemistry

SP3-FAIMS Chemoproteomics for High Coverage Profiling of the Human Cysteinome


We report a new cysteine chemoproteomic method, termed SP3-FAIMS chemoproteomics, which enables rapid and high coverage analysis of the human cysteinome. By combining enhanced cysteine biotinylation with SP3 sample decontamination and FAIMS online fraction, we identified in aggregate 34,225 unique cysteines found on 7,243 proteins. Showcasing the versatility of our method, integration with the isoTOP-ABPP workflow enabled the high throughput discovery of cysteines labelled by electrophilic compounds.


Thumbnail image of 2020-Yan-et-al-ChemRxiv.pdf
download asset 2020-Yan-et-al-ChemRxiv.pdf 1 MB [opens in a new tab]

Supplementary material

Thumbnail image of 2020-Yan-et-al-SI.pdf
download asset 2020-Yan-et-al-SI.pdf 0.80 MB [opens in a new tab]
Thumbnail image of Table S1.xlsx
download asset Table S1.xlsx 34 MB [opens in a new tab]
Table S1
Thumbnail image of Table S2.xlsx
download asset Table S2.xlsx 8 MB [opens in a new tab]
Table S2
Thumbnail image of Table S3.xlsx
download asset Table S3.xlsx 43 MB [opens in a new tab]
Table S3
Thumbnail image of Table S4.xlsx
download asset Table S4.xlsx 23 MB [opens in a new tab]
Table S4
Thumbnail image of Table S5.xlsx
download asset Table S5.xlsx 0.18 MB [opens in a new tab]
Table S5
Thumbnail image of Table S6.xlsx
download asset Table S6.xlsx 0.77 MB [opens in a new tab]
Table S6