Abstract
We report the application of a highly versatile and
engineerable novel sensor platform to monitor biologically significant
and toxic metal ions in live human Caco-2 enterocytes. The extended
conjugation between the fluorescent porphyrin core and metal ions via
aromatic phenylphosphonic acid tethers generates a unique turn off
and turn on fluorescence and, in addition, shifts in absorption and
emission spectra for zinc, cobalt, cadmium and mercury. The reported
fluorescent probes p-H8TPPA and m-H8TPPA can monitor a wide
range of metal ion concentrations via fluorescence titration and also
via fluorescence decay curves. Cu and Zn-induced turn off
fluorescence can be differentially reversed by the addition of common
chelators. Both p-H8TPPA and m-H8TPPA readily pass the
mammalian cellular membrane due to their amphipathic character as
confirmed by confocal microscopic imaging of living enterocytes.