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Kinetic Analysis of the Hydrolysis of Pentose-1-Phosphates Through Apparent Nucleoside Phosphorolysis Equilibrium Shifts

15 October 2020, Version 1
Working Paper
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

Ask what an equilibrium can do for you:
Hydrolysis of pentose-1-phosphates leads to an apparent increase of the equilibrium conversion in nucleoside phosphorolysis reactions. This information can be leveraged via equilibrium thermodynamics to determine the hydrolysis kinetics of in situ generated sugar phosphates, which are known to be elusive and difficult to quantify.

Keywords

nucleoside
nucleoside phosphorylase
enyme
sugar phosphate
hydrolysis
equilibrium
UV spectroscopy

Supplementary materials

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P1P hydrolysis SI
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Now Published

Kinetic Analysis of the Hydrolysis of Pentose‐1‐phosphates through Apparent Nucleoside Phosphorolysis Equilibrium Shifts** [opens in a new tab]
Felix Kaspar, Peter Neubauer, Anke Kurreck journal article
ChemPhysChem , Volume 22, Issue 3
Online publication date: Dec 04, 2020

Version History

Oct 15, 2020 Version 1

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The content is available under CC BY NC ND 4.0 [opens in a new tab]

DOI

10.26434/chemrxiv.13090244.v1
D O I: 10.26434/chemrxiv.13090244.v1 [opens in a new tab]

Author’s competing interest statement

A. K. is CEO of the biotech company BioNukleo GmbH. F. K. is a scientist at BioNukleo GmbH and P. N. is a member of the advisory board. These affiliations constitute no conflict of interest with the results presented and discussed in this report.

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