A Multiuse Nanopore Platform with Disposable Paper Analytical Device for the Detection of Heavy Meatal Ions

28 September 2020, Version 1
This content is a preprint and has not undergone peer review at the time of posting.


The pollution of heavy metal ions within the environmental is a global problem. The rapid and precise removal of these contaminants can be aided by identifying and quantifying the composition of the sample. It is therefore crucial to develop effective portable analytical techniques to determine the levels of heavy metal contamination. Paper-based analytical devices (PADs) offer a low cost method making them an excellent platform for onsite environmental sensors. Here we demonstrate how a PAD can be integrated into a multi-use Nanopore platform. The PAD was functionalised with different recognition ligands, who’s surface charge densities varied in the presence of an analyte. The surface of the PAD was placed in contact with a Nanopore which exhibited Ion Current Rectification (ICR). The extent of ICR, was dependent upon the PAD’s surface charge, and the presence of the analyte of interest i.e. the ICR phenomena was exaggerated or diminished indicating the presence of the metal ion in solution. We demonstrate the potential of PAD-ICR using a PAD functionalised with a peptide aptamer specific for nickel ions. Allowing the detection of nickel(II) as low as 0.25 μM even in the presence of other metal ions. After any measurement, the Nanopore surface can be wiped clean, and reused.


heavy metal ions

Supplementary materials

PAD ICR supplementary prerpint


Comments are not moderated before they are posted, but they can be removed by the site moderators if they are found to be in contravention of our Commenting Policy [opens in a new tab] - please read this policy before you post. Comments should be used for scholarly discussion of the content in question. You can find more information about how to use the commenting feature here [opens in a new tab] .
This site is protected by reCAPTCHA and the Google Privacy Policy [opens in a new tab] and Terms of Service [opens in a new tab] apply.