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Abstract
Human eccrine sweat contains numerous biomarkers which can provide information on health,
performance, and aging. Non-invasive collection and measurement of biomarkers has become
especially important in recent times given viral outbreaks like SARS-CoV-2. In the current study
we describe a method of sweat collection from palmar surfaces in participants via surface capture
using glass beads and the resulting analysis of biomarkers from very low volumes of sweat using
liquid chromatography mass spectrometry with selected ion monitoring. Study participants
underwent a cognitive and physical stress task with easy and hard conditions with sweat being
collected after each task. Resulting analysis found a signal for 22 steroid biomarkers and we
report detailed information on selected biomarkers, given their applicability to timely real-world
exemplars, including cortisol, dehydroepiandrosterone, allopregnanolone, estrone, aldosterone,
and 20a/b-dihydrocortisone.
